Study of the chemical composition of Urochloa brizantha using the SPAD index, neural networks, multiple linear models, principal components and cluster analysis
Autores

Flávia Fernanda Simili, Karolaynny Rhayana Silva Barbosa, Jeferson Garcia Augusto, Leonardo Sartori Menegatto, Gabriela Geraldi Mendonça, Pedro Mielli Bonacim, Flávia Maria de Andrade Gimenes, Rodrigo Pelicioni Savegnago

Resumo

The objectives of this study were to explore the relationship between plant variables using
correlation and principal component analysis; to explore the chemical composition patterns in a
subgroup of plants using cluster analysis; and to compare the prediction ability between a linear
model using only the SPAD index as a predictor with multiple linear regression and neural
networks with the SPAD index, morphological and climatic measurements as predictors of the
chemical composition of Urochloa brizantha leaves and stems. The experimental design was in
blocks (three blocks) with five treatments, totaling 15 experimental units. Chlorophyll measurements and forage sampling were performed every 28 d. The variables used in the statistical
analysis were: percentage of leaves, stems and dead plant material; plant height; relative
chlorophyll (SPAD); percentage of acid detergent fibre in leaves and stems (ADF.L, ADF.S);
percentage of neutral detergent fibre in leaves and stems (NDF.L, NDF.S); lignin percentage in
leaves and stems (LIG.L, LIG.S); and nitrogen content in leaves and stems (N.L, N.S). The climatic
variables were monthly average minimum and maximum temperatures and monthly rainfall. The
correlation between SPAD with N.L and N.S was 0.56 and 0.49, respectively, and between N.L
with N.S was 0.87. The correlation between the observed and predicted responses using simple
linear regression, with SPAD as the predictor, ranged from 0.198 for ADF.L to 0.577 for N.L.
However, the correlations ranged from 0.497 for LIG.L to 0.759 for N.S when multiple regression
was used with other predictors, besides SPAD. The prediction accuracy using neural networks
ranged from 0.501 for LIG.L to 0.863 for N.S and was higher than multiple regression for all
characteristics except LIG.L and LIG.S. Principal component analysis efficiently condensed the
most important information of the 13 original variables measured in the plants into three

Keywords:
Fibrous components
Chlorophyll indices
Multivariate analysis
Nitrogen
Tropical grasses

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