In vitro ovicidal and larvicidal activity of Psidium cattleianum Sabine leaves against gastrointestinal nematodes of naturally infected sheep

  • M. L. S. T. Piza Universidade Estadual Paulista, Departamento de Física e Química, FEIS, SP, Brasil.
  • A. Féboli Universidade Estadual Paulista, Departamento de Física e Química, FEIS, SP, Brasil.
  • J. G. Augusto Universidade Estadual Paulista, Departamento de Biologia e Zootecnia, FEIS, SP, Brasil
  • L. A. Anjos Universidade Estadual Paulista, Departamento de Biologia e Zootecnia, FEIS, SP, Brasil
  • A. C. Laurentiz Universidade Estadual Paulista, Departamento de Biologia e Zootecnia, FEIS, SP, Brasil
  • V. A. Royo Universidade Estadual de Montes Claros, Campus Universitário Professor Darcy Ribeiro, MG, Brazil
  • F. Q. Alvarenga Universidade Estadual de Montes Claros, Campus Universitário Professor Darcy Ribeiro, MG, Brazil
  • R. S. Laurentiz Universidade Estadual Paulista, Departamento de Física e Química, FEIS, SP, Brasil.

Resumo

Gastrointestinal nematodes can affect sheep productivity due severe weight loss, anemia and sequential dead that cause in animals with high level of infection. Infection control is generally performed with synthetics anthelmintic; however, the indiscriminate use of these drugs culminates in the emergence of resistant nematodes. Thus, alternatives to the use of synthetic anthelmintic have been proposed, such as the inclusion of medicinal plants in animal feed. In this context, the objective of the study was evaluate the anthelmintic activity of the leaves of medicinal plant Psidium cattleianum Sabine (araçá) through of its hydroalcoholic extract using in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The extract showed good in vitro ovicidal and larvicidal activity with IC50 values of 0.55 mg/mL for egg hatching inhibition, 0.20 mg/mL for larval development inhibition and efficiency greater than 80% in inhibition of larval migration in all concentrations evaluated (IC50 < 0.19 mg/mL). Phytochemical analysis detected higher concentrations saponins, flavonoids, cardiac glycosides, anthraquinones, and tannins in the extract. Our results demonstrated that the Psidium cattleianum Sabine leaves exhibits in vitro anthelmintic activity, suggesting that, beyond its other medicinal properties, this plant can also be an ally for gastrointestinal nematodes control in sheep.

Referências

ABONGWA, M.; MARTIN, R.J.; ROBERTSON, A.P. A brief review on the mode of action of antinematodal drugs. Acta Veterinaria, v. 67, p. 137-152, 2017. https://doi.org/10.1515/acve-2017-0013

AGUERRE, S.; JACQUIET, P.; BRODIER, H.; BOURNAZEL, J.P.; GRISEZ, C.; PREVÓT, F.; MICHOT, L.; FIDELLE, F.; ASTRUC, J.M.; MORENO, C.R. Resistance to gastrointestinal nematodes in dairy sheep: Genetic variability and relevance of artificial infection of nucleus rams to select for resistant ewes on farms. Veterinary Parasitology, v. 256, p. 16-23, 2018. https://doi.org/10.1016/j.vetpar.2018.04.004

ALONSO-DÍAZ, M.A.; TORRES-ACOSTA, J.F.; SANDOVAL-CASTRO, C.A.; AGUILAR-CABALLERO, A.J.; HOSTE, H. In vitro larval migration and kinetics of exsheathment of Haemonchus contortus larvae exposed to four tropical tanniniferous plant extracts. Veterinary Parasitology, v. 153, p. 313-319, 2008. https://doi.org/10.1016/j.vetpar.2008.01.042

ALVARENGA, F.C.; MOTA, B.C.F.; LEITE, M.N.; FONSECA, J.M.S.; OLIVEIRA, D.A.; ROYO, V.A.; SILVA, M.L.A.; ESPERANDIM, V.; BORGES, A.; LAURENTIZ, R.S. In vivo analgesic activity, toxicity and phytochemical screening of the hydroalcoholic extract from the leaves of Psidium cattleianum Sabine. Journal of Ethnopharmacology, v. 150, p. 280-284, 2013. https://doi.org/10.1016/j.jep.2013.08.044

BARONE, C.D.; ZAJAC, A.M.; MANZI-SMITH, L.A.; HOWELL, A.B.; REED, J.D.; KRUEGER, C.G.; PETERSSON, K.H. Anthelmintic efficacy of cranberry vine extracts on ovine Haemonchus contortus. Veterinary Parasitology, v. 253, p. 122-129, 2018. https://doi.org/10.1016/j.vetpar.2018.02.016

COLES, G.C.; BAUER, C.; BORGSTEEDE, F.H.M.; GEERTS, S.; KLEI, T.R.; TAYLOR, M.A.; WALLER, P.J. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, v. 44, p. 35-44, 1992. https://doi.org/10.1016/0304-4017(92)90141-u

CORTES-MORALESA, J.A.; OLMEDO-JUÁREZ, A.; TREJO-TAPIA, G.; GONZÁLEZ-CORTAZAR, M.; DOMÍNGUEZ-MENDOZA, B.E.; MENDOZA-DE-GIVES, P.; ZAMILPA, A. In vitro ovicidal activity of Baccharis conferta Kunth against Haemonchus contortus. Experimental Parasitology, v. 197, p. 20–28, 2019. https://doi.org/10.1016/j.exppara.2019.01.003

D’ADDABBO, T.; CARBONARA, T.; LEONETTI, P.; RADICCI, V.; TAVA, A.; AVATO, P. Control of plant parasitic nematodes with active saponins and biomass from Medicago sativa. Phytochemistry Reviews, v. 10, p. 503-519, 2011. https://doi.org/10.1007/s11101-010-9180-2

DEBIAGE, R. R.; GONÇALVES, F. F.; PEREIRA, A. R.; SILVA, R. G.; YOSHIHARA, E.; PEIXOTO, E. T. M. Anthelmintic potential of Psidium guajava in sheep. Planta Medica, Leipzig, v. 82, p. 1-381, 2016. https://doi.org/10.1055/s-0036-1596981

DESOTI, V.C.; MALDANER, C.L.; CARLETTO, M.S.; HEINZ, A.A.; COELHO, M.S.; PIATI, D.; TIUMAN, T.S. Triagem fitoquímica e avaliação das atividades antimicrobiana e citotóxica de plantas medicinais nativas da região oeste do estado do Paraná. Arquivos Ciência e Saúde, v. 15, p. 3-13, 2011. https://doi.org/10.25110/arqsaude.v15i1.2011.3686

FALEIRO, J.H.; GONÇALVES, R.C.; SANTOS, M.N.G.; SILVA, D.P.; NAVES, P.L.F.; MALAFAIA, G. The Chemical Featuring, Toxicity, and Antimicrobial Activity of Psidium cattleianum (Myrtaceae) Leaves. New Journal of Science, v. 2016, p. 1-8, 2016. https://doi.org/10.1155/2016/7538613

FÉBOLI, A.; LAURENTIZ, A.C.; SOARES, S.C.; AUGUSTO, J.G.; ANJOS, L.A.; MAGALHÃES, L.G.; LAURENTIZ, R.S. Ovicidal and larvicidal activity of extracts of Opuntia ficus-indica against gastrointestinal nematodes of naturally infected sheep. Veterinary Parasitology, v. 226, p. 65-68, 2016. https://doi.org/10.1016/j.vetpar.2016.06.030

HARBORNE, J.B. Phytochemical Methods: A guide to modern techniques of plant analysis. 3rd ed. Netherlands: Springer Science & Business Media, 1998. 286 p.

HERNÁNDEZ-VILLEGAS, M.M.; BORGES-ARGÁEZ, R.; RODRIGUEZ-VIVAS, R.I.; TORRES-ACOSTA, J.F.J.; MÉNDEZ-GONZALEZ, M.; CÁCERES-FARFAN, M. Ovicidal and larvicidal activity of the crude extracts from Phytolacca icosandra against Haemonchus contortus. Veterinary Parasitology, v. 179, p. 100-106, 2011. https://doi.org/10.1016/j.vetpar.2011.02.019

HO, R.; VIOLETTE, A.; CRESSEND, D.; RAHARIVELOMANANA, P.; CARRUPT, P.A.; HOSTETTMANN, K. Antioxidant potential and radical-scavenging effects of flavonoids from the leaves of Psidium cattleianum grown in French Polynesia. Natural Product Research, v. 26, p. 274-277, 2012. https://doi.org/10.1080/14786419.2011.585610

JUN, N.J.; MOSADDIK, A.; MOON, J.Y.; KI-CHANG, J.; DONG-SUN, L.; AHN, K.S.; CHO, S.K. Cytotoxic Activity of β-Caryophyllene Oxide Isolated from Jeju Guava (Psidium cattleianum Sabine). Records of Natural Products, v. 5, p. 242, 2011.

KANOJIYA, D.; SHANKER, D.; SUDAN, V.; JAISWAL, A.K.; PARASHAR, R. In vitro and in vivo efficacy of extracts of leaves of Eucalyptus globulus on ovine gastrointestinal nematodes. Parasitology Research, v. 114, p. 141-148, 2015. https://doi.org/10.1007/s00436-014-4169-1

KATIKI, L.M.; FERREIRA, J.F.S.; GONZALEZ, J.M.; ZAJAC, A.M.; LINDSAY, D.S.; CHAGAS, A.C.S.; AMARANTE, A.F.T. Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity. Veterinary Parasitology, v. 18, p. 192-218, 2013. https://doi.org/10.1016/j.vetpar.2012.09.030

KATIKI, L.M.; GOMES, A.C.P.; BARBIERI, A.M.E.; PACHECO, P.A.; RODRIGUES, L.; VERÍSSIMO, C.J.; GUTMANISA, G.; PIZA, A.M.; LOUVANDINI, H.; FERREIRA, J.F.S. Terminaliacatappa: Chemical composition, in vitro and in vivo effects on Haemonchus contortus. Veterinary Parasitology, v. 246, p. 118-123, 2017. https://doi.org/10.1016/j.vetpar.2017.09.006

KLONGSIRIWET, C.; QUIJADA, J.; WILLIAMS, A.R.; MUELLER-HARVEY, I.; WILLIAMSON, E.M.; HOSTE, H. Synergistic inhibition of Haemonchus contortus exsheathment by flavonoid monomers and condensed tannins. International Journal for Parasitology: Drugs and Drug Resistance, v. 5, p. 127-134, 2015. https://doi.org/10.1016/j.ijpddr.2015.06.001

MARIE-MAGDELEINE, C.; HOSTE, H.; MAHIEU, M.; VARO, H.; ARCHIMEDE, H. In vitro effects of Cucurbita moschata seed extracts on Haemonchus contortus. Veterinary Parasitology, v. 161, p. 99-105, 2009. https://doi.org/10.1016/j.vetpar.2008.12.008

MARIE-MAGDELEINE, C.; UDINO, L.; PHILIBERT, L.; BOCAGE, B.; ARCHIMEDE, H. In vitro effects of Musa x paradisiaca extracts on four developmental stages of Haemonchus contortus. Research in Veterinary Science, v. 96, p. 127-132, 2014. https://doi.org/10.1016/j.rvsc.2013.12.004

MENGISTU, G.; HOSTE, H.; KARONEN, M.; SALMINEN, J.P.; PELLIKAAN, W.F. The in vitro anthelmintic properties of browse plant species against Haemonchus contortus is determined by the polyphenol content and composition. Veterinary Parasitology, v. 237, p. 110-116, 2017. https://doi.org/10.1016/j.vetpar.2016.12.020

OLIVEIRA, A.F.; JUNIOR, L.M.C.; LIMA, A.S.; SILVA, C.R.; RIBEIRO, M.N.; MESQUISTA, J.W.; ROCHA C.Q.; TANGERINA, M.M.P.; VILEGAS, W. Anthelmintic activity of plant extracts from Brazilian savanna. Veterinary Parasitology, v. 236, p. 121-127, 2017. https://doi.org/10.1016/j.vetpar.2017.02.005

PLOEGER, H.W.; ANTONIS, A.F.G.; VERKAIK, J.C.; VELLEMA, P.; BOKMA-BAKKER, M.H. Perceptions and actions of Dutch sheep farmers concerning worm infections. Veterinary Parasitology, v. 229, p. 150-158, 2016. http://dx.doi.org/10.1016/j.vetpar.2016.10.012

RABEL, B.; MCGREGOR, R.; DOUCH, P.G.C. Improved bioassay for estimation of inhibitory effects of ovine gastrointestinal mucus and anthelmintic on nematode larval migration. International Journal for Parasitology, v. 24, p. 671-676, 1994. https://doi.org/10.1016/0020-7519(94)90119-8

RAJESWARI, V.D. Anthelmintic activity of plants: a review. Research Journal of Phytochemistry, v. 8, p. 57-63, 2014. https://doi.org/10.3923/rjphyto.2014.57.63

SANTOS, F.O.; LIMA, H.G.; SANTOS, N.S.S.; SERRA, T.M.; UZEDA, R.S.; REIS, I.M.A.; BOTURA, M.B.; BRANCO, A.; BATATINHA, M.J.M. In vitro anthelmintic and cytotoxicity activities the Digitaria insularis (Poaceae). Veterinary Parasitology, v. 245, p. 48-54, 2017. https://doi.org/10.1016/j.vetpar.2017.08.007

TORRES-ACOSTA, J.F.J.; MENDOZA-DE-GIVES, P.; AGUILAR-CABALLERO, A.J.; CUÉLLAR-ORDAZ, J.A. Anthelmintic resistance in sheep farms: update of the situation in the American continent. Veterinary Parasitology, v. 189, p. 89-96, 2012. https://doi.org/10.1016/j.vetpar.2012.03.037

VARGAS-MAGAÑA, J.J.; TORRES-ACOSTA, J.F.J.; AGUILAR-CABALLERO, A.J.; SANDOVAL-CASTRO, C.A.; HOSTE, H.; CHAN-PÉREZ, J.I. Anthelmintic activity of acetone–water extracts against Haemonchus contortus eggs: Interactions between tannins and other plant secondary compounds. Veterinary Parasitology, v. 206, p. 322-327, 2014. https://doi.org/10.1016/j.vetpar.2014.10.008

WILLIAMS, A.R.; ROPIAK, H,M.; FRYGANAS, C.F.; DESRUES, O.; HARVEY, I.M.; THAMBORG, S.M. Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum. Parasites & Vectors, v. 7, p. 518, 2014. https://doi.org/10.1186/s13071-014-0518-2

Publicado
21-10-2019
Como Citar
Piza, M., Féboli, A., Augusto, J., Anjos, L., Laurentiz, A., Royo, V., Alvarenga, F., & Laurentiz, R. (2019). In vitro ovicidal and larvicidal activity of Psidium cattleianum Sabine leaves against gastrointestinal nematodes of naturally infected sheep. Boletim De Indústria Animal, 76, 1-8. https://doi.org/10.17523/bia.2019.v76.e1450
Seção
SANIDADE ANIMAL