Autores
Adna Crisléia Rodrigues Monção de Lima, Thiago Pereira Motta, Mariana Santos de Miranda, Juliana Rodrigues Pozzi Arcaro, Cláudia Rodrigues Pozzi
California mastitis test in the diagnostic of subclinical mastitis
Resumo
California mastitis test no diagnóstico da mastite subclínica
Abstract
Milk production in Brazil is undoubtedly one of the
most important Brazilian agroindustrial complex. Moves large sums of money, the dairy industry employs
millions of the people, having potential to provide the domestic and foreign markets.
Besides surpassing year by year the index production. The quality of milk is
increasingly demanded by consumers and there are bonus programs for milk with
low somatic cell counts, which reveal, indirectely, the udder sanity. Mastitis,
the udder inflamation, is the main factor that substantially compromises the
milk quality. Several methods can diagnose the incidence of subclinical
mastitis in dairy herds. One these methods, the California Mastitis Test (CMT)
has as advantages being practical, low cost and the results are immediately
available. The CMT method consists of adding the anionic neutral detergent to a
milk sample in order to disrupt milk somatic cell membranes and release nucleic
material. The viscousity formed by this reaction allows estimating the number
of somatic cells (immunity cells) presents in the milk. According to the degree
of gelatinization obtained in this reaction, the interpretation of the scores varies
from zero, no viscosity, to three crosses, highly viscous. This study was aimed
to evaluate the CMT of eight dairy herds of different farms in Sao Paulo state, described by the letters A
to H. The scores 1, 2 and 3 were considered positive for subclinical mastitis,
while 0 was negative. The results were
determined in relative frequency (%). It is evident that the
herd D is the most affected by subclinical mastitis, because of the greater
number of CMT positive (60%). This may be due to the mismanagement and poor
conditions of milking. The properties C, F and G require greater
attention, as the result of CMT could corroborate the presence of pathogenic
microorganisms and infected cows can quickly transmit the infection to the
healthy ones. Note that the farms A, B and H are the ones with the largest
number of CMT negative, that is, with cows with better conditions of udder sanity. Over all it is found that CMT consists of an
effective test for the detection of subclinical mastitis.
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