Débora Cavallaro Machado, Sylvia Lúcia Gonçalves Garcia Gazzeta, Camila De Miranda E Silva Chaves, Ricardo Lopes Dias Da Costa, Keila Maria Roncato Duarte
Screening of Toxoplasma gondii in ovine by immunosorbent assay
Toxoplasmosis is one of the
most frequent zoonosis around the world and occurs by the contact of host to Toxoplasma gondii parasite. In humans,
the transmission can be congenital, when pregnant woman are exposed to the
parasite and the fetus can develop micro and macro encephalitis, mental
retardation, abortion, etc. In animals, transmition can be congenital or can be
done by saliva, milk or semen containing Toxoplasma
gondii. Ovine are potential transmitters, where the parasite cists are
found in animal tissue for long periods of time. Disease diagnoses are needed
to avoid economic losses and human health problems. Despite immunofluorescence
is the most used test to diagnose T.
gondii, ELISA tests were used in this study to screen animals in ovine
herd, at IZ. For those, 134 animals from Morada Nova and Santa Inês breeds were
tested using the IDEXX Toxoplasma gondii test
kit, using sera as biological sample. Blood was taken from jugular vein and
centrifugation was performed. Sera were separated and kept frozen. Presence of T. gondii was verified in ELISA test and
reading at Biotech microplate reader, analyzed by Xchek 3.3 software. O.D.
showed 27 animals with the presence of T.
gondii. Only one animal was diagnosed “suspect” and new sample will be run.
ELISA test showed to be fast, easy and reliable in the farm. Once the amount of
sera used is minimum (1 ul), same blood sample can be used for several
screening diseases, avoiding animal stress. For these tested animals, around
20% present T. gondii, which is lower
than São Paulo State average (around 40 %), meaning a good management of ovine
herd. A monitoring of T. gondii in
ovine herd is essential to guarantee quality meat and quality management
practices for food safety and sustainability.
Identificação de toxoplasma gondii em ovino por ensaio imunoenzimático