José Brites Neto; Keila Maria Roncato Duarte (orientadora)
Brazilian spotted fever (BSF) is an anthropozoonosis with epidemiological characteristics of high endemicity and high lethality. The present study was designed to perform an epidemiologic diagnosis of infectivity for Rickettsia rickettsii in a population of 3,548 adult free living ticks, collected in 83 acarologist researches, identified as Amblyomma cajennense (2,355) and Amblyomma dubitatum (1,193). Seasonality was verified of the population of the species of ticks collected during the period comprised between July, 2009 and June, 2010. Among the adult ticks, 702 were submitted to the hemolymph test and 2,197 were subjected to dissection in order to remove salivary glands for DNA extraction and evaluation of gltA-PCR and ompA-PCR. Areas evaluated for BSF presented indexed between 1.2% and 8.8% of positive samples for gltA-PCR , and were all negative for ompA2-PCR, in species Amblyomma cajennense and Amblyomma dubitatum, in the areas of alert and risk located in the city of Americana, São Paulo. In the context of sustainable animal production, the objectives of this project contributed to the mapping of the distribution of the species Amblyomma cajennense and Amblyomma dubitatum of ticks and for the evaluation of the infection prevalence in the same ticks for Rickettsia spp., ensuing a better understanding of the real interferences on the biotic potentials in preservation areas contiguous to areas dedicated to husbandry and measuring eventual risks to zootechnical research performed in the Research Center for Cattle Raising and Milk of the Zootechnical Institute, located in Americana.
KEY WORDS: Amblyomma cajennense. Amblyomma dubitatum. Brazilian
spotted fever. PCR. Rickettsia spp. Infectivity